Ly6g 抗体 (FITC)
Quick Overview for Ly6g 抗体 (FITC) (ABIN114271)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Absorption / Emission: 495 nm / 528 nm
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纯化方法
- Protein G chromatography
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亚型
- IgG2b
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anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 FACS, WB, IP, IHC (fro), Func, IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (APC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE-Cy7)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 FITC
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibodyLy6g 适用: 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (Biotin)Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE)Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE
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应用备注
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Flow cytometry (1,2,3). Western blot (5).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1 - 0. 5 µg of antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: CBA/JCell Concentration : 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsIsotypic Control: FITC Rat IgG2bCell Source Percentage of cells stained above control: Thymus 1. 5%Whole Blood Monocytes 87. 2%Bone Marrow Macrophages 90. 0%(see picture below)Strain Distribution by Flow Cytometry Analysis: Procedure: see aboveCell Concentration: 1x10e6 cells per test
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
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别名
- Ly6G / GR1 Neutrophil Marker
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背景
- GR-1 is a 25-30 kDa cell surface antigen and is expressed on myeloid cells but not lymphoid or erythroid cells. The expression of the Gr-1 antigen increases with granulocyte maturation (3) as shown by the distinct populations of bone-marrow cells this monoclonal antibody labels: negative, low positive and high positive. Expression is transient on cells of monocytic lineage (3).Synonyms: Gr-1 Granulocyte marker
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基因ID
- 10090
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UniProt
- P35461
抗原
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