L-Selectin 抗体 (PE)
Quick Overview for L-Selectin 抗体 (PE) (ABIN114236)
抗原
See all L-Selectin (SELL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Protein G Chromatography
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免疫原
- Mouse B cell Lymphoma, 38C-14
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亚型
- IgG2a
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anti-Selectin L (SELL) (AA 301-372) antibody
SELL 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) (AA 1-100) antibodySELL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibody (APC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal LT-TD180 APC
anti-Selectin L (SELL) (AA 83-186) antibodySELL 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 8C8B7 unconjugated
anti-Selectin L (SELL) (AA 109-346) antibodySELL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibodySELL 适用: 人, Cow FACS, IP, ICC 宿主: 小鼠 Monoclonal IVA94 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal DREG56 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal Mel-14 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), Func, ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
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应用备注
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Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: PE Rat IgG2aCell Source: Percentage of cells stained above control: Thymus: 89. 1%Spleen: 34. 8%Lymph Node: 88. 5%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: BALB/c, CBA/J, C3H/He, C57BL/6, AKR
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- This product is photosensitive and should be protected from light
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- L-Selectin (SELL) (Selectin L (SELL))
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别名
- CD62L / L-Selectin
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物质类
- Chemical
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背景
- L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro 2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
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基因ID
- 20343
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NCBI登录号
- NP_035476
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UniProt
- P18337
抗原
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