L-Selectin 抗体 (Selectin L) (Biotin)

Details for Product anti-SELL Antibody No. ABIN114231, 供应商: Log in to see
抗原
  • CD62L
  • LAM1
  • LECAM1
  • LEU8
  • LNHR
  • LSEL
  • LYAM1
  • PLNHR
  • TQ1
  • A.11
  • L-selectin
  • LECAM-1
  • SELE
  • AI528707
  • Lnhr
  • Ly-22
  • Ly-m22
  • Lyam-1
  • Lyam1
  • selectin L
  • selectin, lymphocyte
  • SELL
  • Sell
适用
化学剂
632
41
39
14
3
1
1
1
宿主
大鼠
352
257
92
13
2
1
1
克隆类型 (克隆位点)
单克隆 ()
标记
This L-Selectin antibody is conjugated to Biotin
90
79
43
40
18
18
16
16
14
12
9
8
8
8
8
8
8
7
6
6
6
6
6
6
4
4
4
4
3
2
2
2
2
1
应用范围
Flow Cytometry (FACS)
596
202
158
131
91
79
48
42
19
14
12
12
10
9
7
7
5
4
3
3
2
2
2
2
2
2
2
1
1
1
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
免疫原 Mouse B cell Lymphoma, 38C-14 Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
克隆位点 MEL14
亚型 IgG2a
特异性 This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
纯化方法 Protein G Chromatography
别名 CD62L / L-Selectin (SELL Antibody 摘要)
物质类 Chemical
背景 L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
基因ID 20343
NCBI登录号 NP_035476
UniProt P18337
应用备注 Flow cytometry. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2aCell Source: Percentage of cells stained above controlThymus: 59. 8%Spleen: 66. 3%Lymph Node: 75. 8%
限制 仅限研究用
浓度 0.1 mg/mL
缓冲液 PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Flow Cytometry (FACS) image for anti-Selectin L (SELL) antibody (Biotin) (ABIN114231) Rat anti CD62L (L-Selectin, LECAM-1) MEL14
背景 Siegelman, Cheng, Weissman, Wakeland: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, 1990 (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, 1987 (PubMed).

Jalkanen, Bargatze, de los Toyos, Butcher: "Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells." in: The Journal of cell biology, Vol. 105, Issue 2, pp. 983-90, 1987 (PubMed).

Fink, Gallatin, Reichert, Butcher, Weissman: "Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation." in: Nature, Vol. 313, Issue 5999, pp. 233-5, 1985 (PubMed).

Reichert, Gallatin, Butcher, Weissman: "A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes." in: Cell, Vol. 38, Issue 1, pp. 89-99, 1984 (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).

您还需要查找其他产品吗?