CD8 抗体 (PE)
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Quick Overview for CD8 抗体 (PE) (ABIN114168)
抗原
See all CD8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This anti-CD8a (Ly 2) monoclonal antibody reacts with a protein of approximately 30 kDa found on mouse thymocytes and mouse cytotoxic/suppressor T cells. It does not bind to mouse helper/inducer T cells. It binds to T lymphocytes from all mouse strains regardless of phenotypic expression (ie. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype.). It can be used to investigate the role of T cells in models for infectious disease, autoimmunity, transplantation tolerance and fundamental aspects of immunology. (1) It can also be useful to identify/eliminate cytotoxic or suppressor T lymphocytes in vivo or in vitro. Strains Tested: C57BL/6, BALB/c, AKR/J, C3H/He Positive: : C57BL/6, BALB/c, AKR/J, C3H/He Negative: None.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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免疫原
- Mouse Ly-2 thymocytes. Donor: (LOU X DA) F1 rat. Fusion Partner: Y3/Ag1.2.3.
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亚型
- IgG2b
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) (AA 135-235) antibodyCD8 适用: 人 IHC 宿主: 兔 Monoclonal 008R unconjugated Recombinant Antibody
anti-CD8 (CD8) antibodyCD8 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (Biotin)CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PE)CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE
anti-CD8 (CD8) antibody (APC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC
anti-CD8 (CD8) antibody (PerCP)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP
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应用备注
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Flow Cytometry Analysis (see Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend cells to a concentration of 2x10e7 cells/ml Media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test. 4. To each tube add 2. 0 µg of this Ab per 10e6 cells. 5. Vortex to ensure through mixing of antibody and cells. 6. Incubate tubes at 4°C for 30 minutes. (It is recommended that the tubes are protectedfrom light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C in Media B. 8. Resuspend the cell pellet in 50 µl ice cold Media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 ul of 2 M sodium azide in 100 mls. )B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 ulof 2 M sodium azide in 100 mls. )Results - Tissue DistributionMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 2. 0 µg/10e6 cellsIsotypic Control: PE Rat IgG2b,kPercentage of cells stained above control: Thymus 66. 2%Spleen 19. 6 %Lymph node 14. 0%
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This antibody is photosensitive and should be protected from light.
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- CD8
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别名
- CD8
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背景
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
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基因ID
- 12525
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NCBI登录号
- NP_001074579
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UniProt
- P01731
抗原
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