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JUP 抗体

This anti-JUP antibody is a 小鼠 单克隆 antibody detecting JUP in WB, IF, IHC (p), IP 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, 小鸡, Cow 和 斑马鱼.
产品编号 ABIN112236
发货至: 中国

Quick Overview for JUP 抗体 (ABIN112236)

抗原

See all JUP 抗体
JUP (Junction Plakoglobin (JUP))

适用

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人, 小鼠, 大鼠, 小鸡, Cow, 斑马鱼

宿主

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小鼠

克隆类型

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单克隆

标记

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This JUP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

PG 5-1
  • 特异性

    PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells), Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells, adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).

    交叉反应 (详细)

    Species reactivity (tested):Human, Bovine, Rat, Mouse, Chicken and Zebrafish.

    纯化方法

    Affinity Chromatography on Protein A

    免疫原

    Plakoglobin, "band 5" protein from Bovine snout epidermis

    亚型

    IgG2b
  • 应用备注

    Immunoblotting. Immunoprecipitation. Immunofluorescence Microscopy: 1/5 (1 h at RT). Immunohistochemistry on Frozen Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in 1 mL distilled water

    缓冲液

    Final solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    JUP (Junction Plakoglobin (JUP))

    别名

    Junction Plakoglobin

    背景

    Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP

    基因ID

    3728

    NCBI登录号

    NP_002221

    UniProt

    P14923

    途径

    Cell-Cell Junction Organization, Maintenance of Protein Location
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