C3 抗体
Quick Overview for C3 抗体 (ABIN112153)
抗原
See all C3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kDa fragment of C3c). Disorders Specifically Detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with Positive Control anti C3b-beta, (H11, Cat. No. BM2132) and Negative Control anti-C3a (H13, Cat. No. BM2131). Activated, deposited C3b bears C3b-alpha and C3b-beta, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein A.
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免疫原
- Human complement component C3
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亚型
- IgG1
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anti-Complement Component 3 (C3) (AA 1000-1326) antibody
C3 适用: 人 WB, IHC, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1551-1663) antibodyC3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (fro), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1534-1644) antibodyC3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5F9 unconjugated
anti-Complement Component 3 (C3) (C-Term) antibodyC3 适用: 人 WB, IP, ICC, IF, IHC (p), ISub 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 23-300) antibodyC3 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 965-1303) antibodyC3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1534-1645) antibodyC3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5F9 unconjugated
anti-Complement Component 3 (C3) antibodyC3 适用: 小鼠 WB, IP, FACS, IF, IA 宿主: 大鼠 Monoclonal 11H9 unconjugated
anti-Complement Component 3 (C3) (AA 966-1313) antibodyC3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 3 (C3) (AA 1353-1382) antibodyC3 适用: 人 WB 宿主: 小鼠 Monoclonal 567CT11-2-3 unconjugated
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应用备注
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ELISA. Immunobloting (Western). Immunohistochemistry on Frozen Sections (also after Fixation with Paraformaldehyde): Working Dilution: 1/10 in PBS, pH 7.4. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in 1 mL distilled water
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缓冲液
- Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- C3 (Complement Component 3 (C3))
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别名
- Complement C3
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背景
- The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
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基因ID
- 718
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NCBI登录号
- NP_000055
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UniProt
- P01024
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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