CD15 抗体
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北京 101111
Quick Overview for CD15 抗体 (ABIN112000)
抗原
See all CD15 (FUT4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity chromatography.
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免疫原
- Human granulocytes.
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亚型
- IgM
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anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 144-389) antibody
FUT4 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 264-497) antibodyFUT4 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 251-295) antibodyFUT4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 200-300) antibodyFUT4 适用: 人 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 199-302) antibodyFUT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B11B4 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 1-147) antibodyFUT4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6C5A3 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-158 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IHC, IF 宿主: 小鼠 Monoclonal Leu-M1-same as MMA unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 4E10 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (APC)FUT4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-158 APC
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应用备注
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ELISA. Immunohistochemistry on frozen sections: 1 μg/mL (1: 200) for tissue staining. Antigen Distribution: Isolated cells: VIM-C6 is heterogenously expressed on granulocytes and on monoblastoidprecursor cells. Tissue sections: VIM-C6 is positive on myeloid precursor cells in the bone marrow. Crossreactions with glandular epithelial cells, astrocytes and Sternberg-Reed cells were reportedfor CD 15.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.2 mg/mL
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缓冲液
- PBS buffer pH 7.2 with 0.02 % sodium azide as preservative and 10 mg/mL BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C (lyophilized) or reconstituted in aliquots at -20 °C.
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- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
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别名
- CD15
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背景
- CD15 is a carbohydrate antigen expressed mainly on mature granulocytes and monocytes. CD15 is also called Lewis X, X-hapten, lacto-N-fucopentaose III or stage-specific embryonic antigen (SSEA). The reactivity is preferentially with granulocytes and to a lesser degree with monocytes. CD15 is expressed on immature bone marrow cells and leukemic cells of the myelo-monocytic lineage, and sometimes on lymphocytic leukemia cells. VIM-C6 facilitates the differentiation of granulocytes from other blood cells. It is also useful for phenotyping cell subpopulations in myeloic leukemias and for enrichment or elimination of myeloid cells. This antibody reacts with granulocytes, monoblastoid precursor cells.Synonyms: Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten
抗原
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