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CD31 抗体 (Biotin)

PECAM1 适用: 小鼠 FACS, IHC (p), IHC (fro) 宿主: 大鼠 Monoclonal ER-MP12 Biotin
产品编号 ABIN111979
发货至: 中国
  • 抗原 See all CD31 (PECAM1) 抗体
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    适用
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    小鼠
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    大鼠
    克隆类型
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    单克隆
    标记
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    This CD31 antibody is conjugated to Biotin
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应 (详细)
    Species reactivity (tested):Mouse.
    纯化方法
    Affinity Chromatography.
    免疫原
    Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.
    克隆位点
    ER-MP12
    亚型
    IgG2a
    Top Product
    Discover our top product PECAM1 Primary Antibody
  • 应用备注
    Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).
    浓度
    0.6 mg/mL
    缓冲液
    PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.
    储存液
    Kathon CG
    注意事项
    This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store (in aliquots) at -20 °C.
  • Mateos, Landeira-Abia, Fafián-Labora, Fernández-Pernas, Lesende-Rodríguez, Fernández-Puente, Fernández-Moreno, Delmiro, Martín, Blanco, Arufe: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." in: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).

    Morikawa, Kajimura, Nakamura, Hishiki, Nakanishi, Yukutake, Nagahata, Ishikawa, Hattori, Takenouchi, Takahashi, Ishii, Matsubara, Kabe, Uchiyama, Nagata, Gadalla, Snyder, Suematsu: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).

    Sakamoto, Geisel, Kim, Wyatt, Sellers, Smiley, Cooper, Russell, Rhoades: "Fibrinogen regulates the cytotoxicity of mycobacterial trehalose dimycolate but is not required for cell recruitment, cytokine response, or control of mycobacterial infection." in: Infection and immunity, Vol. 78, Issue 3, pp. 1004-11, (2010) (PubMed).

    Larghero, Venè, Minghelli, Travaini, Morini, Ferrari, Pfeffer, Noonan, Albini, Benelli: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." in: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).

    Li, Sainson, Shi, Leek, Harrington, Preusser, Biswas, Turley, Heikamp, Hainfellner, Harris: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." in: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).

    Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).

    Li, Otsu, Murphy, Raymond: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).

  • 抗原
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    别名
    CD31 / PECAM1 (PECAM1 产品)
    别名
    CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody
    背景
    Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule
    基因ID
    10090
    UniProt
    Q08481
    途径
    Regulation of Actin Filament Polymerization
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