CD31 抗体 (Biotin)
(7 references)Quick Overview for CD31 抗体 (Biotin) (ABIN111979)
抗原
See all CD31 (PECAM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography.
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免疫原
- Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.
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亚型
- IgG2a
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 601-680) antibody
PECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, 犬 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC (p), IHC (f) 宿主: 山羊 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-382) antibodyPECAM1 适用: 人 FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 681-738) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow FACS, WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-315) antibodyPECAM1 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 9E6E12 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 29-738) antibodyPECAM1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D2-1A5 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 兔, Cynomolgus FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal JC-70A unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal C31-3 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 625-738) antibodyPECAM1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal PECAM1-3527 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠, 兔, Cynomolgus FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal C31-7 unconjugated
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应用备注
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Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).
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浓度
- 0.6 mg/mL
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缓冲液
- PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.
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储存液
- Kathon CG
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注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store (in aliquots) at -20 °C.
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: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." in: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).
: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).
: "Fibrinogen regulates the cytotoxicity of mycobacterial trehalose dimycolate but is not required for cell recruitment, cytokine response, or control of mycobacterial infection." in: Infection and immunity, Vol. 78, Issue 3, pp. 1004-11, (2010) (PubMed).
: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." in: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).
: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." in: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).
: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).
: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).
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: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." in: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- CD31 / PECAM1
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背景
- Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule
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基因ID
- 10090
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UniProt
- Q08481
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途径
- Regulation of Actin Filament Polymerization
抗原
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