Angiotensin I Converting Enzyme 1 抗体
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Quick Overview for Angiotensin I Converting Enzyme 1 抗体 (ABIN111943)
抗原
See all Angiotensin I Converting Enzyme 1 (ACE) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Angiotensin converting Enzyme (ACE), denatured. The epitope recognized is localized on the NH2-terminal side of the molecule. Topographically the degree of expression correlates with the proportion of smooth muscle cells and elastic fibres of the vessel wall. ACE is predominantely expressed by small muscular arteries and by arterioles, whereas small and large veins do not usually express ACE. However, no ACE could be detected in the endothelial cells of kidney. Constitutively ACE is expressed on endothelial cells in different diseases like hypertension, myocardial infarction, sarcoidosis, diabetes.
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纯化方法
- Protein A chromatography.
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免疫原
- Purified ACE from human lung and kidney.
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亚型
- IgG1
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anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 1160-1306) antibody
ACE 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 651-864) antibodyACE 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (fro), IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 814-1071) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 801-900) antibodyACE 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, ICC, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 702-715), (Middle Region) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 592-701) antibodyACE 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-270) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 592-701) antibodyACE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A4 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-182) antibodyACE 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3H7E4 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) antibodyACE 适用: 人 ELISA 宿主: 小鼠 Monoclonal 9B9 unconjugated
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应用备注
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Suitable for Immunohistochemistry on Paraffin sections: 8 μg/mL (1: 50), microwavepretreatment for antigen retrieval is recommended. Suggested positive control: Human lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7min. at 700Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS15. Counterstain with Mayer's hemalum For further information and details see technicalinformation
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2 containig 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.1 % Kathon as a preservative.
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储存液
- Kathon CG
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注意事项
- This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
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别名
- ACE / CD143
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背景
- ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.Synonyms: Angiotensin-converting enzyme, DCP, DCP1, Dipeptidyl carboxypeptidase I
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基因ID
- 1636
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NCBI登录号
- NP_000780
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UniProt
- P12821
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
抗原
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