CD31 抗体
(1 reference)Quick Overview for CD31 抗体 (ABIN111924)
抗原
See all CD31 (PECAM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for CD31.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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免疫原
- Human CD31
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亚型
- IgG1
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 601-680) antibody
PECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, 犬 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC (f), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-382) antibodyPECAM1 适用: 人 FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 681-738) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow FACS, WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-315) antibodyPECAM1 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 9E6E12 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 29-738) antibodyPECAM1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D2-1A5 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠, 兔, Cynomolgus FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal C31-7 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 625-738) antibodyPECAM1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal PECAM1-3527 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal C31-3 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 兔, Cynomolgus FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal JC-70A unconjugated
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应用备注
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Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/200-1/400). Immunohistochemistry on Paraffin Sections: 8 μg/mL (1/50). Proteinase K pretreatment forantigen retrieval is recommended. Suggested Positive Control: Human placenta. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat antimouse IgG+IgM (H+L) minimal-cross reaction to human) for 1 h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min. 12. Wash in PBS13. Counterstain with Mayer's hemalum
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限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL distilled water to 0.4 mg/mL.
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缓冲液
- Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!
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: "Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells." in: Journal of lipid research, Vol. 49, Issue 5, pp. 1103-12, (2008) (PubMed).
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: "Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells." in: Journal of lipid research, Vol. 49, Issue 5, pp. 1103-12, (2008) (PubMed).
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- CD31 / PECAM1
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背景
- CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). The CD31 antigen is found on cells lining and within the vasculature, and plays a role in the adhesive interaction between adjacent endothelial cells as well as between leukocytes and endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule
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基因ID
- 5175, 9606
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UniProt
- P16284
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途径
- Regulation of Actin Filament Polymerization
抗原
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