CD163 抗体 (Biotin)
(2 references)Quick Overview for CD163 抗体 (Biotin) (ABIN111916)
抗原
See all CD163 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Clone 5C6 FAT recognizes CD163, a membrane glycoprotein on human monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution on isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Antigen Distribution Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, andcortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody BM4022 (clone 25F9) which is not the case in other tissues. Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Negative In Pig.
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纯化方法
- Affinity Chromatography
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免疫原
- Human CD163. The epitope has not been further characterized.
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亚型
- IgG1
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anti-CD163 (CD163) (AA 1001-1121) antibody
CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1001-1121) antibody (FITC)CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 IHC, ELISA, ISt, Coat, StM 宿主: 小鼠 Monoclonal M130-2162 unconjugated
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal M130-1210 unconjugated
anti-CD163 (CD163) (AA 42-259) antibodyCD163 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 7G5E2 unconjugated
anti-CD163 (CD163) (AA 697-972) antibodyCD163 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) antibodyCD163 适用: 人 WB, FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal GHI-61 unconjugated
anti-CD163 (CD163) (AA 47-201) antibodyCD163 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1092-1109), (C-Term) antibodyCD163 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 693-939) antibodyCD163 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Tested for immunohistochemistry (IHC) and ELISA, has been described to work in FACS andWestern blots. Approximate working dilution for IHC: Frozen sections: 0.5 μg/mL (1: 400)Paraffin sections: 1 μg/mL, Proteinase K pretreatment for antigen retrieval recommended. Suggested positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.2 mg/mL
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缓冲液
- Stock Solution containing PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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: "Apoptotic cell death and its relationship to gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 13, Issue 23, pp. 3183-8, (2007) (PubMed).
: "Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3183-94, (2007) (PubMed).
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: "Apoptotic cell death and its relationship to gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 13, Issue 23, pp. 3183-8, (2007) (PubMed).
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- CD163
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别名
- CD163
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背景
- CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
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基因ID
- 9332
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NCBI登录号
- NP_004235
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UniProt
- Q86VB7
抗原
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