ITGA5 抗体 (Biotin)
Quick Overview for ITGA5 抗体 (Biotin) (ABIN111906)
抗原
See all ITGA5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e. Detects Human granulocytes, monocytes and platelets. Cross reactivity: unknown. X-6 detects an antigen of 154-180 kDa by Immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes, positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.
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交叉反应 （详细）
- Species reactivity (tested):Human: Granulocytes, Monocytes, Platelets.
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纯化方法
- Affinity Chromatography
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免疫原
- Human Monocytes.
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亚型
- IgG1
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anti-Integrin, alpha 5 (ITGA5) (AA 42-263) antibody
ITGA5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 1021-1099) antibodyITGA5 适用: 人, 小鼠 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) antibodyITGA5 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 10F6 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 111-253) antibodyITGA5 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6F6C1 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 111-253) antibodyITGA5 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8D1G12 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 220-472) antibodyITGA5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (Internal Region) antibodyITGA5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 750-1000) antibodyITGA5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 575-602) antibodyITGA5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB20677 unconjugated
anti-Integrin, alpha 5 (ITGA5) (AA 649-900) antibodyITGA5 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: use 10 μL stock solution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water
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浓度
- 0.4 mg/mL
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缓冲液
- Stock solution contains PBS, pH 7.2 with 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.
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注意事项
- Dilute only prior to immediate use
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储存条件
- 4 °C
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储存方法
- Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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- ITGA5 (Integrin, alpha 5 (ITGA5))
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别名
- CD49e / ITGA5
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背景
- The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5
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基因ID
- 3678
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NCBI登录号
- NP_002196
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UniProt
- P08648
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途径
- Integrin Complex
抗原
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