S100A8 抗体 (C-Term) (Biotin)
Our Local Distributor
北京 101111
Quick Overview for S100A8 抗体 (C-Term) (Biotin) (ABIN111893)
抗原
See all S100A8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
特异性
- S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
-
交叉反应 （详细）
- Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).
-
纯化方法
- Affinity Chromatography
-
免疫原
- Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.
-
亚型
- IgG1
-
-
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibody
S100A8 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibodyS100A8 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-93) antibodyS100A8 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (C-Term) antibodyS100A8 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) antibodyS100A8 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 25-89) antibodyS100A8 适用: 人, 小鼠 ELISA, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A8 (S100A8) (AA 1-89) antibodyS100A8 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.
-
限制
- 仅限研究用
-
-
-
溶解方式
- Restore in 0.5 mL distilled water to make stock solution.
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
-
储存液
- Thimerosal (Merthiolate)
-
注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- This product is photosensitive and should be protected from light
-
储存条件
- 4 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
-
-
- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
-
别名
- S100A8 / Calgranulin-A / MRP8
-
背景
- MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
-
基因ID
- 9606
-
UniProt
- P05109
-
途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
-
