His Tag 抗体
Quick Overview for His Tag 抗体 (ABIN1112988)
抗原
See all His Tag 抗体适用
宿主
标记
应用范围
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序列
- HHHHHH
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特异性
- Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.
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免疫原
- A synthetic peptide 6 × His conjugated to KLH.
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亚型
- IgG1
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
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应用备注
- WB (1:5000), IP (1:100), IF (1:2000). Other applications have not been tested. The optimal dilutions should be determined by end user.
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限制
- 仅限研究用
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状态
- Liquid
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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储存条件
- -20 °C
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储存方法
- Store at -20 °C for 1 year. Or aliquot and store at -80 °C for long term. Avoid repeated freeze and thaw cycles.
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有效期
- 12 months
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- His Tag
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物质类
- Tag
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背景
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.
抗原
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