(1 reference)-
- 抗原 See all Integrin beta 3 (ITGB3) 抗体
- Integrin beta 3 (ITGB3)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Integrin beta 3 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD61 PE is recommended for use in flow cytometry for identification of human Platelets.
- 克隆位点
- C17
- 亚型
- IgG1
- Top Product
- Discover our top product ITGB3 Primary Antibody
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anti-Integrin beta 3 (ITGB3) (AA 27-120) antibody
ITGB3 适用: 人, 小鼠 WB, FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 3 (ITGB3) (AA 134-376) antibodyITGB3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 3 (ITGB3) antibodyITGB3 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin beta 3 (ITGB3) (AA 27-179) antibodyITGB3 适用: 人 WB, FACS, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 3E8A6 unconjugated
anti-Integrin beta 3 (ITGB3) (AA 385-490) antibodyITGB3 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ITGB3-2145 unconjugated
anti-Integrin beta 3 (ITGB3) antibody (FITC)ITGB3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal VIPL2 FITC
anti-Integrin beta 3 (ITGB3) antibody (PerCP)ITGB3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal VIPL2 PerCP
anti-Integrin beta 3 (ITGB3) (AA 610-718) antibodyITGB3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 3 (ITGB3) (AA 734-760) antibodyITGB3 适用: 人 WB, FACS, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 3 (ITGB3) (AA 135-377) antibodyITGB3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).
- 样品收集
- 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD61 PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0, 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0, 09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Evaluation of a CD61 MoAb method for enumeration of platelets in thrombocytopenic patients and its impact on the transfusion decision-making process." in: Transfusion, Vol. 41, Issue 10, pp. 1212-6, (2001) (PubMed).
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: "Evaluation of a CD61 MoAb method for enumeration of platelets in thrombocytopenic patients and its impact on the transfusion decision-making process." in: Transfusion, Vol. 41, Issue 10, pp. 1212-6, (2001) (PubMed).
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- 抗原
- Integrin beta 3 (ITGB3)
- 别名
- CD61 (ITGB3 产品)
- 别名
- BDPLT16 antibody, BDPLT2 antibody, CD61 antibody, GP3A antibody, GPIIIa antibody, GT antibody, INGRB3 antibody, integrin antibody, INTB3 antibody, GPIIIA antibody, cd61 antibody, gp3a antibody, itgb3 antibody, itgb3.2 antibody, itgb3.1 antibody, integrin subunit beta 3 antibody, integrin beta 3 antibody, integrin subunit beta 3 L homeolog antibody, integrin beta 3a antibody, integrin beta 3b antibody, ITGB3 antibody, Itgb3 antibody, itgb3.L antibody, itgb3a antibody, itgb3b antibody
- 背景
- This clone has been clustered to CD61 in the fourth international Workshop on Human White Cell differentiation Antigens. The antibody is specific to the 110kDa platelet glycoprotein gp IIIa and inhibits platelet aggregation and activation induced by thrombin and collagen. The platelet glycoprotein is the integrin -3 chain of the vitronectin receptor and GP IIb/IIIa. The antigen is present on platelets and megakaryocytes.
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex
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