(1 reference)-
- 抗原 See all CD15 (FUT4) 抗体
- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD15 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD15 FITC, is recommended for use in flow cytometry for identification of neutrophils, monoblastoid precursor cells of the myeloid lineage, eosinophils, and some monocytes, but not on basophils and lymphocytes.
- 克隆位点
- MCS-1
- 亚型
- IgG3
- Top Product
- Discover our top product FUT4 Primary Antibody
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anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 264-497) antibody
FUT4 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 144-389) antibodyFUT4 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 251-295) antibodyFUT4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-158 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 200-300) antibodyFUT4 适用: 人 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 1-147) antibodyFUT4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 6C5A3 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 199-302) antibodyFUT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B11B4 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal Leu-M1-same as MMA unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC)FUT4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-158 FITC
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin)FUT4 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-158 Biotin
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD15 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing Stabilizing Solution, PBS pH 7,4 – 0,2
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Epigenetics modifications and Subclinical Atherosclerosis in Obstructive Sleep Apnea: The EPIOSA study." in: BMC pulmonary medicine, Vol. 14, pp. 114, (2015) (PubMed).
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: "Epigenetics modifications and Subclinical Atherosclerosis in Obstructive Sleep Apnea: The EPIOSA study." in: BMC pulmonary medicine, Vol. 14, pp. 114, (2015) (PubMed).
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- 抗原
- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
- 别名
- CD15 (FUT4 产品)
- 别名
- AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody
- 背景
- The CD15 antigen is the X-hapten on lacto-N-fucose pentaosyl lll, which is expressed on circulating granulocytes or tissue granulocytes as well as neutrophils and eosinophils. The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage.
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