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ENO2/NSE 抗体

ENO2 适用: 人 WB, IHC (p), IHC (fro), EIA 宿主: 小鼠 Monoclonal 5G10 unconjugated
产品编号 ABIN110966
发货至: 中国
  • 抗原 See all ENO2/NSE (ENO2) 抗体
    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This ENO2/NSE antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    This antibody is specific for Human Neuron Specific Enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma. Reacts with a 45 kDa band corresponding to NSE on Immunoblots of brain extracts.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Affinity Chromatography on Protein G.
    免疫原
    Native NSE protein from Human brain.
    克隆位点
    5G10
    亚型
    IgG2b
    Top Product
    Discover our top product ENO2 Primary Antibody
  • 应用备注
    ELISA. Immunohistology on Paraffin Sections: 1/100-1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Control: Brain cortex.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS with 0.09 % sodium azide as preservative.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
    别名
    Neuron Specific Enolase (ENO2 产品)
    别名
    ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody
    背景
    Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase
    基因ID
    2026
    NCBI登录号
    NP_001966
    UniProt
    P09104
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