ENO2/NSE 抗体
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北京 101111
Quick Overview for ENO2/NSE 抗体 (ABIN110966)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for Human Neuron Specific Enolase (NSE), a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma. Reacts with a 45 kDa band corresponding to NSE on Immunoblots of brain extracts.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography on Protein G.
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免疫原
- Native NSE protein from Human brain.
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亚型
- IgG2b
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibody
ENO2 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Immunohistology on Paraffin Sections: 1/100-1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections(Sodium Citrate buffer pH 6.0 is recommended for this purpose). Positive Control: Brain cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS with 0.09 % sodium azide as preservative.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- Neuron Specific Enolase
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背景
- Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase
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基因ID
- 2026
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NCBI登录号
- NP_001966
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UniProt
- P09104
抗原
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