GFP
适用: Aequorea victoria
WB, ELISA, IHC, IP, FACS, FM
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: 1/10000. Western Bloting: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
限制
仅限研究用
缓冲液
PBS, 0.03 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
抗原
GFP
(Green Fluorescent Protein (GFP))
别名
GFP
背景
GFP (Green Fluorescence Protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein