Complement C4A/C4B (Chain alpha 2) 抗体
This 小鼠 单克隆 anti-not_set antibody (Clone 12D11) specifically detects not_set in WB, EIA 和 IHC (fro). The antibody is reactive with 人, Cow, 猴 和 Pig samples.
产品编号 ABIN1106789
发货至:
中国
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Quick Overview for Complement C4A/C4B (Chain alpha 2) 抗体 (ABIN1106789)
抗原
Complement C4A/C4B
适用
人, Cow, 猴, Pig
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
12D11
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抗原表位
- Chain alpha 2
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特异性
- The monoclonal antibody 12D11 (also known as clone M4d3) recognizes an epitope on the alpha 2 chain of human C4d. The antibody 12D11 recognizes C4, C4b and C4d.
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交叉反应 （详细）
- Species reactivity (tested):Human, Baboon, Rhesus monkey, Bovine, Swine.
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纯化方法
- Protein G
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亚型
- IgG1
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anti-Complement C4A/C4B (Internal Region) antibody
适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement C4A/C4B (Center) antibody适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
Western blot: The typical starting working dilution is 1:50
Immunoassay
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.02 % Sodium Azide, 0.1 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2 - 8 °C.
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- Complement C4A/C4B
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背景
- Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the α-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35 % shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4
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基因ID
- 720
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NCBI登录号
- NP_001239133
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UniProt
- P0C0L4
抗原
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