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- 抗原 See all FASL 抗体
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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适用
- 人, 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This FASL antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with mouse FasL of B6, C3H and MRL mice as well as Balb/c and DBA/1mice, but does not detect gld-type mouse FasL mutant by flow cytometric analysis. We have tested the reactivity on flow cytometry using mouse cell line W40LFL, which is overexpressor of mouse FasL extracellular region (See ref. 11 for more information about this cell line).
- 交叉反应 （详细）
- Species reactivity (tested):Mouse. Weakly with human.
- 纯化方法
- Protein A agarose
- 免疫原
- W40LFL cells, which is overexpressed mouse FasL extracellular region
- 克隆位点
- FLIM58
- 亚型
- IgG
- Top Product
- Discover our top product FASL Primary Antibody
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anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-281) antibody
FASL 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-200) antibodyFASL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 102-280) antibodyFASL 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-278) antibodyFASL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 103-281) antibodyFASL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 82-145) antibodyFASL 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) antibodyFASL 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2G9-G8 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal NOK-1 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal NOK-1 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- 50 tests in 1 mL volume of 10 mM phosphate buffer ( pH 8.0), 0.3 M NaCl and 0.09 % NaN3, 1 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2 - 8 °C.
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- 抗原
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- 别名
- CD178 / Fas Ligand (FASL 产品)
- 别名
- ALPS1B antibody, APT1LG1 antibody, APTL antibody, CD178 antibody, CD95-L antibody, CD95L antibody, FASL antibody, TNFSF6 antibody, fasl antibody, Fas-L antibody, Faslg antibody, Tnfsf6 antibody, gld antibody, Apt1Lg1 antibody, Fasl antibody, FASLG antibody, zgc:162027 antibody, Fas ligand antibody, Fas ligand L homeolog antibody, Fas ligand (TNF superfamily, member 6) antibody, FASLG antibody, faslg.L antibody, Fasl antibody, Faslg antibody, faslg antibody
- 背景
- Fas ligand (FasL/CD178/CD95L) is a type II-membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
- 基因ID
- 356
- NCBI登录号
- NP_000630
- UniProt
- P48023
- 途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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