RANKL 抗体
Quick Overview for RANKL 抗体 (ABIN1103902)
抗原
See all RANKL (TNFSF11) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human sRANK Ligand
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human sRANK Ligand.
Type of Immunogen: Cell extract -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7419 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sRANKL (LS-C104397) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
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别名
- TNFSF11 / RANKL / TRANCE
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背景
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Name/Gene ID: TNFSF11
Family: TNF
Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL -
基因ID
- 8600
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UniProt
- O14788
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途径
- NF-kappaB Signaling
抗原
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