RPA2 抗体 (pSer122)
Quick Overview for RPA2 抗体 (pSer122) (ABIN106543)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer122
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Supplier Product No.
- 600-401-447
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Supplier
- Rockland
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原理
- RFA2 phospho S122 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against yeast RFA2.
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产品特性
- Synonyms: rabbit anti-RFA2 pS122 antibody, RFA-2, RFA 2, Replication factor A protein 2, RF-A protein 2, Replication protein A 36 kDa subunit, DNA-binding protein BUF1, BUF-1, YNL312W, N0368
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 110-135 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G1/S transition and is dephosphorylated at mitosis.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Replication Protein A2, 32kDa (RPA2) (AA 1-270) antibody
RPA2 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 7C12 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 1H10 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 9A1 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 1-267) antibodyRPA2 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (N-Term) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 101-200) antibodyRPA2 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity-purified antibody was tested for use in western blotting against RFA2 containing cell lysates and by ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.
Western Blot Dilution: 1:500 - 1:1000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:8,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.54 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RFA2
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背景
- Background: RFA2 binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). It stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
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基因ID
- 855404, 132475
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UniProt
- P26754
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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