PAK1/2/3 抗体 (pThr423)
Quick Overview for PAK1/2/3 抗体 (pThr423) (ABIN105217)
抗原
See all PAK1/2/3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr423
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Supplier Product No.
- 600-401-413
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Supplier
- Rockland
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原理
- PAK 1/2/3 phospho T423 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human PAK (p21-GTPase Activated Protein Kinase).
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产品特性
- Synonyms: rabbit anti-PAK pT423 antibody, rabbit anti-PAK1/2/3 pT423 antibody, PAK1, PAK-1, PAK 1, Alpha PAK, p21-activated kinase 1, p65-PAK, PAK2, PAK-2, PAK 2, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Gamma PAK, PAK3, PAK-3, PAK 3, Beta PAK, CDC42/RAC effector kinase PAK-B, p21-activated kinase 3
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (Thr402), (Thr421), (Thr423) antibody
PAK1/2/3 适用: 人 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (Thr402), (Thr421), (Thr423) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (pThr423) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠, Cow, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (AA 391-440) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinases 1/2/3 (PAK1/2/3) (AA 111-160), (pSer144) antibodyPAK1/2/3 适用: 人, 大鼠, 小鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This phospho specific polyclonal antibody was tested by Dot blot and ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immunoprecipitation. Lysates from Jurkat cells or PAK transfected cells may be used as a control. This product has been assayed against 0.1 μg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 is suggested for this product. Less than 0.2 % cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.94 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))
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别名
- PAK 1/2/3
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背景
- Background: The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis. Mammalian PAKs are called 1, 2, 3 or a, g, b respectively. PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae. Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids. PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins. This antibody is specific for the phosphorylated form of PAK 1/2/3. The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
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基因ID
- 5058
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UniProt
- Q13153
抗原
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