NCKAP1 抗体 (Internal Region)
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Quick Overview for NCKAP1 抗体 (Internal Region) (ABIN1049510)
抗原
See all NCKAP1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- NCKAP1
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序列
- TPREYLTSHL EIR
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特异性
- This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.
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亚型
- IgG
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anti-NCK Associated Protein 1 (NCKAP1) antibody
NCKAP1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) antibodyNCKAP1 适用: 人 WB, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) antibodyNCKAP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NCK Associated Protein 1 (NCKAP1) (AA 1-250) antibodyNCKAP1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- NCKAP1 (NCK Associated Protein 1 (NCKAP1))
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别名
- NCKAP1
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背景
- NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1
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基因ID
- 10787, 50884, 58823
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NCBI登录号
- NP_038464, NP_995314
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途径
- RTK signaling, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
抗原
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