Neurotrophin 3 抗体 (N-Term)
Quick Overview for Neurotrophin 3 抗体 (N-Term) (ABIN1049152)
抗原
See all Neurotrophin 3 (NTF3) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
品牌
- IHC-plus™
-
特异性
- Human NTF3 / Neurotrophin 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
-
预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Opossum (100%) Turkey (93%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic 15 amino acid peptide from N-Terminus of human NTF3 / Neurotrophin 3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Opossum (100%), Turkey (93%).
Type of Immunogen: Synthetic peptide
-
-
anti-Neurotrophin 3 (NTF3) (AA 139-257) antibody
NTF3 适用: 人 WB, IHC, ICC, IHC (p), IF 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Neurotrophin 3 (NTF3) (AA 152-184), (C-Term) antibodyNTF3 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal RB14406 unconjugated
anti-Neurotrophin 3 (NTF3) (AA 19-100) antibodyNTF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 130-255) antibodyNTF3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 19-257) antibodyNTF3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 130-255) antibodyNTF3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 1-257) antibodyNTF3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 174-189), (Middle Region) antibodyNTF3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 21-138) antibodyNTF3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurotrophin 3 (NTF3) (AA 21-139) antibodyNTF3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Approved: IHC, IHC-P (5 μg/mL)
-
说明
-
Target Species of Antibody: Human
-
实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, less than 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
-
有效期
- 12 months
-
-
- Neurotrophin 3 (NTF3)
-
别名
- NTF3 / Neurotrophin 3
-
背景
-
Name/Gene ID: NTF3
Family: NGF Beta
Synonyms: NTF3, HDNF, NGF2, Nerve growth factor 2, NGF-2, NT-3, Neurotrophic factor, Neurotrophin 3, Neurotrophin-3 -
基因ID
- 4908
-
途径
- RTK signaling, Neurotrophin Signaling Pathway
抗原
-
