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IL-18 抗体

This anti-IL-18 antibody is a 兔 多克隆 antibody detecting IL-18 in WB, IHC, ELISA, IF 和 FM. Suitable for 小鼠. This Primary Antibody has been cited in 3+ publications.
Rockland
产品编号 ABIN964783
Supplier Product No.: 210-401-323
发货至: 中国

Quick Overview for IL-18 抗体 (ABIN964783)

抗原

See all IL-18 (IL18) 抗体
IL-18 (IL18) (Interleukin 18 (IL18))

适用

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克隆类型

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多克隆

标记

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This IL-18 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • Supplier Product No.

    210-401-323

    Supplier

    Rockland

    原理

    IL-18 Antibody

    交叉反应 (详细)

    This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays.

    产品特性

    Synonyms: rabbit anti-IL-18 antibody, rabbit anti-interleukin-18 antibody, Iboctadekin antibody, IFN gamma inducing factor antibody, IGIF antibody, IL 1 gamma antibody, IL 18 antibody, IL 1g antibody, IL-1F4, IL 18, Interleukin 18, IL18, Interleukin18, IL1 F4, IL1F4, Il18

    纯化方法

    This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology.

    过滤

    Sterile filtered

    免疫原

    Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.

    Immunogen Type: Recombinant Protein

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Mouse IL-18 has been tested in immunohistochemistry and immunofluorescence and is suitable for use in neutralizations, ELISA, and immunoblotting.  Although untested, this reagent may be useful for radioimmunoassays, flow cytometry and immunoprecipitation.  It recognizes the 18,000 MW mature (active) IL-18.  Reactivity in other immunoassays is unknown.

    Neutralization Dilution: User Optimized

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:5,000

    IF Microscopy Dilution: 1:50-1:200

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: None

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Quach, Song, Guo, Li, Maazi, Fung, Sands, OConnell, Restrepo-Vassalli, Chai, Nemecio, Punj, Akbari, Idos, Mumenthaler, Wu, Martin, Hagiya, Hicks, Cui, Liang: "A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice." in: Nature communications, Vol. 10, Issue 1, pp. 5681, (2020) (PubMed).

    Leal-Lasarte, Franco, Labrador-Garrido, Pozo, Roodveldt: "Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 7, pp. 2797-2816, (2017) (PubMed).

    Sakamuri, Valente, Siddesha, Delafontaine, Siebenlist, Gardner, Bysani: "TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis." in: Molecular and cellular endocrinology, Vol. 429, pp. 84-92, (2016) (PubMed).

  • 抗原

    IL-18 (IL18) (Interleukin 18 (IL18))

    别名

    Il18

    背景

    Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1.  IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule.  In both the mature and propeptide forms, IL-18 shows 64 % aa sequence identity from mouse to human.  IL-18 does not appear to show any primary sequence similarity to any other known cytokines.  Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91 % aa sequence identity to mouse IL-18.  Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC.  With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway.  Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.

    基因ID

    16173

    UniProt

    P70380

    途径

    Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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