beta Actin 抗体 (N-Term)
(1 reference)
Quick Overview for beta Actin 抗体 (N-Term) (ABIN1043747)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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Supplier Product No.
- 200-301-e69
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Supplier
- Rockland
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原理
- Beta Actin Antibody
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交叉反应 （详细）
- beta Actin antibody is specific for human and mouse ß Actin protein.
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产品特性
- Synonyms: mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
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纯化方法
- Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Immunogen Type: Conjugated Peptide
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亚型
- IgG2a lambda
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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 2-16) antibodyACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 350-375) antibodyACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 1-25) antibodyACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyKO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated
anti-Actin, beta (ACTB) (AA 1-50) antibodyACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:5000-1:50000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-Beta Actin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).
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: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- ACTB
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背景
- Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
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基因ID
- 11461
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NCBI登录号
- NP_001092
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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