CYBB 抗体
Quick Overview for CYBB 抗体 (ABIN1042596)
抗原
See all CYBB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389
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纯化方法
- Protein G
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免疫原
- Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
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亚型
- IgG1
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应用备注
- Optimal antibody concentration should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 μg/mL.IHC-P: Use a concentration of 5 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol
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说明
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Myeloma, fusion partners: SP2/0
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Purified antibody containing PBS 0.1 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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: "Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58." in: Biochimica et biophysica acta, Vol. 1752, Issue 2, pp. 186-96, (2005) (PubMed).
: "Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16974-80, (1995) (PubMed).
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: "Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58." in: Biochimica et biophysica acta, Vol. 1752, Issue 2, pp. 186-96, (2005) (PubMed).
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- CYBB (Cytochrome B-245, beta Polypeptide (CYBB))
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别名
- NOX2/gp91phox
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背景
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Synonyms:
CGD91-phox antibody,CYBB antibody,Cytochrome b(558) beta chain antibody,Cytochrome b-245 heavychain antibody,gp91-1 antibody,gp91-phox antibody,Heme-binding membrane glycoprotein gp91phox antibody,NADPHoxidase 2 antibody,Neutrophil cytochrome b 91 kDa polypeptide antibody,NOX2 antibody,p22 phagocyte B-cytochromeantibody,Superoxide-generating NADPH oxidase heavy chain subunit antibody -
基因ID
- 21577
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OMIM
- 300481
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UniProt
- Q01062
抗原
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