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CYBB 抗体

Cited in 2+ publications. This 小鼠 单克隆 anti-CYBB antibody (Clone 54-1) specifically detects CYBB in WB, IF, IHC (p) 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN1042596
发货至: 中国
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Quick Overview for CYBB 抗体 (ABIN1042596)

抗原

See all CYBB 抗体
CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CYBB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

克隆位点

54-1
  • 特异性

    This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389

    纯化方法

    Protein G

    免疫原

    Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)

    亚型

    IgG1
  • 应用备注

    Optimal antibody concentration should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 μg/mL.IHC-P: Use a concentration of 5 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol

    说明

    Myeloma, fusion partners: SP2/0

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Purified antibody containing PBS 0.1 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Baniulis, Nakano, Nauseef, Banfi, Cheng, Lambeth, Burritt, Taylor, Jesaitis: "Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58." in: Biochimica et biophysica acta, Vol. 1752, Issue 2, pp. 186-96, (2005) (PubMed).

    Burritt, Quinn, Jutila, Bond, Jesaitis: "Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16974-80, (1995) (PubMed).

  • 抗原

    CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

    别名

    NOX2/gp91phox

    背景

    Synonyms:
    CGD91-phox antibody,CYBB antibody,Cytochrome b(558) beta chain antibody,Cytochrome b-245 heavychain antibody,gp91-1 antibody,gp91-phox antibody,Heme-binding membrane glycoprotein gp91phox antibody,NADPHoxidase 2 antibody,Neutrophil cytochrome b 91 kDa polypeptide antibody,NOX2 antibody,p22 phagocyte B-cytochromeantibody,Superoxide-generating NADPH oxidase heavy chain subunit antibody

    基因ID

    21577

    OMIM

    300481

    UniProt

    Q01062
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