alpha 2 Macroglobulin 抗体 (Biotin)
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- 抗原 See all alpha 2 Macroglobulin (A2M) 抗体
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This alpha 2 Macroglobulin antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-106-207-0100
- Supplier
- Rockland
- 原理
- Alpha-2-Macroglobulin Antibody Biotin Conjugated
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified alpha-2-MACROGLOBULIN [Human Plasma].
- 产品特性
- Synonyms: goat anti-Alpha-2-Macroglobulin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody
- 纯化方法
- Anti-Alpha-2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: alpha-2-MACROGLOBULIN [Human Plasma]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product A2M Primary Antibody
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- 应用备注
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Application Note: Anti-Alpha-2-Macroglobulin has been tested by western blot and is suitable to be assayed against 1.0 μg of alpha-2-MACROGLOBULIN in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 is suggested for this product.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- A2M (A2M 产品)
- 背景
- Background: Anti-alpha-2-Macroglobulin antibody is secreted into the plasma and belongs to the protease inhibitor I39 family. Anti-alpha-2-Macroglobulin inhibits all proteinases utilizing a ""bait region,"" or specific cleavage site, which traps the proteinase, after which a thioester bond is hydrolyzed and regulates the covalent binding of the protein to proteinase. Anti-alpha-2-Macroglobulin antibody is ideal for investigators interested in Cardiovascular , Protease Inhibitor, or Cell Biology research.
- 基因ID
- 2
- NCBI登录号
- NP_000005
- UniProt
- P01023
- 途径
- Lipid Metabolism
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