alpha 2 Macroglobulin 抗体
(1 reference)
Quick Overview for alpha 2 Macroglobulin 抗体 (ABIN103762)
抗原
See all alpha 2 Macroglobulin (A2M) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-101-207-0100
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Supplier
- Rockland
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原理
- Alpha-2-Macroglobulin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma].
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产品特性
- Synonyms: goat anti-Alpha-2-Macroglobulin Antibody, A2M antibody, Alpha 2 M antibody, Alpha 2M antibody, CPAMD5 antibody, DKFZp779B086 antibody, FWP007 antibody, S863 7 antibody
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纯化方法
- Anti-alpha-2-MACROGLOBULIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: a2-Macroglobulin [Human Plasma]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibody
A2M 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibodyA2M 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1053-1214) antibodyA2M 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1216-1467) antibodyA2M 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1-1474) antibodyA2M 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 641-730) antibodyA2M 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, ELISA, IP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人, Pig, 兔, 犬 IHC, ELISA, IP, IEP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1-1474) antibodyA2M 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-alpha-2-macroglobulin antibody has been tested by western blot and is suitable for ELISA and Immunohistochemistry applications. Anti-alpha-2-MACROGLOBULIN antibody should be optimized by the end user for the specific reactive conditions.
Western Blot Dilution: 1:2,000 - 1:20,000
ELISA Dilution: 1:80,000 - 1:400,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Association of salivary alpha 2-macroglobulin levels and clinical characteristics in type 2 diabetes." in: Journal of diabetes investigation, Vol. 7, Issue 2, pp. 190-6, (2017) (PubMed).
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: "Association of salivary alpha 2-macroglobulin levels and clinical characteristics in type 2 diabetes." in: Journal of diabetes investigation, Vol. 7, Issue 2, pp. 190-6, (2017) (PubMed).
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- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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别名
- A2M
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背景
- Background: Alpha-2-Macroglobulin detects Macroglobulin. Alpha-2-MACROGLOBULIN is a large plasma protein found in the blood. It is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. Alpha 2-Macroglobulin is the largest major nonimmunoglobulin protein in plasma. The alpha 2-macroglobulin molecule is synthesized mainly in liver, but also locally by macrophages, fibroblasts, and adrenocortical cells. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha 2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β. No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of Alpha 2 macroglobulin. The concentration of alpha 2 macroglobulin rises 10-fold or more in the nephrotic syndrome when other lower molecular weight proteins are lost in the urine. The loss of alpha 2 macroglobulin into urine is prevented by its large size. The net result is that alpha 2 macroglobulin reaches serum levels equal to or greater than those of albumin in the nephrotic syndrome, which has the effect of maintaining oncotic pressure. Antialpha-2-macroglobulin is ideal for investigators involved in serum component protein research.
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基因ID
- 2
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NCBI登录号
- NP_000005
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UniProt
- P01023
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途径
- Lipid Metabolism
抗原
-
