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GFP 抗体 (Rhodamine)

Name: GFP 抗体 (Rhodamine)
SKU: ABIN100095
Availability: OutOfStock

Rockland 600-100-215 GFP 适用: Aequorea victoria WB, FM, DB, FLISA 宿主: 山羊 Polyclonal Rhodamine

产品编号 ABIN100095

Supplier Product No.: 600-100-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
167

15

11

9
Aequorea victoria
宿主
61

58

35

13

9

5

1

1
山羊
Compatible Secondaries
克隆类型
111

68
多克隆
标记
81

11

10

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Rhodamine
应用范围
144

79

46

46

29

29

25

24

15

13

13

13

12

11

4

4

2

1

1

1

1

1
Western Blotting (WB), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Supplier Product No.

600-100-215

Supplier

Rockland

原理

GFP Antibody Rhodamine Conjugated

交叉反应（详细）

Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

产品特性

Synonyms: goat anti-GFP Antibody Rhodamine Conjugation, TRITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

GFP Rhodamine Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

亚型

IgG

Labeling Ratio

2.22

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应用备注

Application Note: Goat Anti-GFP Rhodamine Conjugated Antibody has been tested by dot blot and western blot and is useful in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Western Blot Dilution: >1:10,000
FLISA Dilution: >1:20,000
IF Microscopy Dilution: 1:500 - 1:2,500
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Anti GFP at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: Anti-GFP antibody is Rhodamine labeled. Conjugated GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt

P42212