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GFP 抗体 (Texas Red (TR))

Name: GFP 抗体 (Texas Red (TR))
SKU: ABIN100090
Availability: OutOfStock

Rockland 600-109-215 GFP 适用: Aequorea victoria WB, FM, FLISA 宿主: 山羊 Polyclonal Texas Red (TR)

(1 reference)

产品编号 ABIN100090

Supplier Product No.: 600-109-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
166

15

11

9
Aequorea victoria
宿主
60

58

35

13

9

5

1

1
山羊
Compatible Secondaries
克隆类型
110

68
多克隆
标记
80

11

10

10

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Texas Red (TR)
应用范围
143

79

46

46

29

28

25

23

15

13

13

13

13

11

4

4

2

1

1

1

1

1
Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Supplier Product No.

600-109-215

Supplier

Rockland

原理

GFP Antibody Texas Red™ Conjugated

交叉反应（详细）

Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

产品特性

Synonyms: goat anti-GFP Antibody Texas Red™ Conjugation, Texas Red™ conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

GFP Texas Red™ Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

亚型

IgG

Labeling Ratio

4.2

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应用备注

Application Note: Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Western Blot Dilution: >1:10,000
FLISA Dilution: >1:20,000
IF Microscopy Dilution: 1:500 - 1:2,500
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store GFP antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Gupta, Verfaillie, Chmielewski, Kren, Eidman, Connaire, Heremans, Lund, Blackstad, Jiang, Luttun, Rosenberg: "Isolation and characterization of kidney-derived stem cells." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 11, pp. 3028-40, (2007) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: GFP Texas Red™ Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt

P42212