Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag)

ABIN3077209 产品详细信息, 供应商: Log in to see
蛋白名称
  • CG12117
  • Dmel\\CG12117
  • SR
  • sptr
  • DDBDRAFT_0188686
  • DDBDRAFT_0191275
  • DDB_0188686
  • DDB_0191275
  • xspr
  • AA409688
  • ENSMUSG00000071345
  • Gm10328
  • SDR38C1
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Tac1r
  • Sepiapterin reductase
  • sepiapterin reductase
  • sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
  • tachykinin receptor 1
  • Sptr
  • sprE
  • CpipJ_CPIJ000511
  • sr
  • spr
  • spre
  • Spr
  • SPR
  • TACR1
  • Tacr1
产品特性
AA 1-261
6
2
1
1
1
1
1
宿主

10
2
1
1
1
1
1
资源
大肠杆菌(E. Coli)
5
4
2
1
1
蛋白类型
Recombinant
标记
His tag
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

序列 MHHHHHHEGGLGRAVCLLTGASRGFGRTLAPLLASLLSPGSVLVLSARNDEALRQLEAELGAERSGLRVVRVPADLGAEAGLQQLLGALRELPRPKGLQRLLLINNAGSLGDVSKGFVDLSDSTQVNNYWALNLTSMLCLTSSVLKAFPDSPGLNRTVVNISSLCALQPFKGWALYCAGKAARDMLFQVLALEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFYDK
Sequence including N-terminal His-tag.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human SPR Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
纯化方法 Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Endotoxin has not been removed. Please contact us if you require endotoxin removal.
质量等级 Crystallography grade
背景 Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
分子量 29.0 kDa Including tag.
UniProt P35270
研究领域 Metabolism, Neurology
途径 Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
说明

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag) (ABIN3077209) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
 image for Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag) (ABIN3077209) Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag) (Image 2)
 image for Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag) (ABIN3077209) Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag) (Image 3)