Aggrecan Protein (ACAN) (AA 20-675)
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- 抗原 See all Aggrecan (ACAN) 蛋白
- Aggrecan (ACAN)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 20-675
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宿主
- 人
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资源
- HEK-293 Cells
- 应用范围
- ELISA, Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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Aggrecan (ACAN) (AA 1-721) protein (GST tag)
ACAN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Aggrecan (ACAN) (AA 56-155) protein (GST tag)ACAN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant human aggrecan (G1-IGD-G2) binds immobilized hyaluronan-biotin (0.1 μg/ml) on a streptavidin plate in a dose dependent manner with a ED50 of 2.5 - 9.0 μg/ml.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 浓度
- 200 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- Aggrecan (ACAN)
- 别名
- Aggrecan (ACAN 产品)
- 别名
- AGC1 Protein, AGCAN Protein, CSPG1 Protein, CSPGCP Protein, MSK16 Protein, SEDK Protein, Agc Protein, Agc1 Protein, Cspg1 Protein, b2b183Clo Protein, cmd Protein, aggrecan Protein, aggrecan core protein Protein, ACAN Protein, Acan Protein, LOC100380783 Protein, AGC Protein
- 背景
- Aggrecan, also known as aggrecan 1, is the major proteoglycan in the articular cartilage. This high molecular weight protein exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. Aggrecan plays an important role in the proper functioning of articular cartilage by providing a hydrated gel structure through its interaction with hyaluronan and link proteins, that endows the cartilage with load-bearing properties. Aggrecan is expressed by chondrocytes and found to be crucial in chondroskeletal morphogenesis during development. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Structurally, aggrecan is composed of two globular structural domains (G1 and G2) at the N-terminal end and one globular domain (G3) at the C-terminal end, separated by a large extended domain (CS) heavily modified with glycosaminoglycans. G1 and G2 domains of aggrecan are separated by the interglobular domain (IGD). Biologically, the G1 domain interacts with hyaluronan acid and link proteins, forming stable ternary complexes in the extracellular matrix (ECM). The G2 domain is homologous to the tandem repeats of G1 and link proteins, and is involved in product processing. G3 makes up the carboxyl terminus of the core protein, and enhances glycosaminoglycan modification and product secretion. Also, the G3 domain links the proteoglycan aggregates to the ECM proteins (fibulins and tenascins). Degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging.
- 分子量
- This 672 amino acid recombinant protein with a C-terminal His tag has a predicted molecular mass of approximately 74.3 kDa. The protein migrates at about 120 kDa by SDS-PAGE in DTT-reducing and non-reducing conditions. The predicted N-terminal amino acid
- 途径
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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