Aggrecan (ACAN) (AA 20-675) (Active) 蛋白

ABIN2666766 产品详细信息, 供应商: Log in to see
蛋白名称
  • AGC1
  • AGCAN
  • CSPG1
  • CSPGCP
  • MSK16
  • SEDK
  • Agc
  • Agc1
  • Cspg1
  • b2b183Clo
  • cmd
  • ARALAR
  • aggrecan
  • solute carrier family 25 (aspartate/glutamate carrier), member 12
  • LOC100380783
  • ACAN
  • Acan
  • AGC
  • SLC25A12
产品特性
AA 20-675
2
1
1
1
1
1
1
1
1
1
1
1
宿主

11
4
4
1
1
资源
HEK-293
17
2
1
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Flow Cytometry (FACS), ELISA
选项
Supplier
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纯度 > 95 % , as determined by Coomassie stained SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平

Less than 0.1 EU per μg of protein as determine by the LAL method.

背景 Aggrecan, also known as aggrecan 1, is the major proteoglycan in the articular cartilage. This high molecular weight protein exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. Aggrecan plays an important role in the proper functioning of articular cartilage by providing a hydrated gel structure through its interaction with hyaluronan and link proteins, that endows the cartilage with load-bearing properties. Aggrecan is expressed by chondrocytes and found to be crucial in chondroskeletal morphogenesis during development. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Structurally, aggrecan is composed of two globular structural domains (G1 and G2) at the N-terminal end and one globular domain (G3) at the C-terminal end, separated by a large extended domain (CS) heavily modified with glycosaminoglycans. G1 and G2 domains of aggrecan are separated by the interglobular domain (IGD). Biologically, the G1 domain interacts with hyaluronan acid and link proteins, forming stable ternary complexes in the extracellular matrix (ECM). The G2 domain is homologous to the tandem repeats of G1 and link proteins, and is involved in product processing. G3 makes up the carboxyl terminus of the core protein, and enhances glycosaminoglycan modification and product secretion. Also, the G3 domain links the proteoglycan aggregates to the ECM proteins (fibulins and tenascins). Degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging.
分子量 This 672 amino acid recombinant protein with a C-terminal His tag has a predicted molecular mass of approximately 74.3 kDa. The protein migrates at about 120 kDa by SDS-PAGE in DTT-reducing and non-reducing conditions. The predicted N-terminal amino acid
研究领域 Stem Cells, Extracellular Matrix
途径 Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
应用备注 Optimal working dilution should be determined by the investigator.
说明

Biological activity: Recombinant human aggrecan (G1-IGD-G2) binds immobilized hyaluronan-biotin (0.1 μg/ml) on a streptavidin plate in a dose dependent manner with a ED50 of 2.5 - 9.0 μg/ml.

限制 仅限研究用
状态 Liquid
溶解方式 For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
浓度 200 μg/mL
缓冲液 0.22 μm filtered protein solution is in PBS.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 -20 °C
储存方法 Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
厂商提供的图像
Flow Cytometry (FACS) image for Aggrecan (ACAN) (AA 20-675) (Active) protein (ABIN2666766) Aggrecan (ACAN) (AA 20-675) (Active) protein
背景 Melin Fürst, Mörgelin, Vadstrup, Heinegård, Aspberg, Blom: "The C-type lectin of the aggrecan G3 domain activates complement." in: PLoS ONE, Vol. 8, Issue 4, pp. e61407, 2013 (PubMed).

Nap, Szleifer: "Structure and interactions of aggrecans: statistical thermodynamic approach." in: Biophysical journal, Vol. 95, Issue 10, pp. 4570-83, 2008 (PubMed).

Roughley, Martens, Rantakokko, Alini, Mwale, Antoniou: "The involvement of aggrecan polymorphism in degeneration of human intervertebral disc and articular cartilage." in: European cells & materials, Vol. 11, pp. 1-7; discussion 7, 2006 (PubMed).

Kiani, Chen, Wu, Yee, Yang: "Structure and function of aggrecan." in: Cell research, Vol. 12, Issue 1, pp. 19-32, 2002 (PubMed).

Doege, Sasaki, Kimura, Yamada: "Complete coding sequence and deduced primary structure of the human cartilage large aggregating proteoglycan, aggrecan. Human-specific repeats, and additional alternatively spliced forms." in: The Journal of biological chemistry, Vol. 266, Issue 2, pp. 894-902, 1991 (PubMed).

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