Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is = 1.0 ng/mL, corresponding to a specific activity of = 1 x 106 units/mg.
FGF2
宿主: 大鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
注意事项
As with any protein, exposing FGF-Basic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
储存条件
-20 °C
储存方法
The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted FGF-Basic is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant murine FGF-basic is a 16.2 kDa protein consisting of 145 amino acid residues.