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Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 22-401) (Active) protein (His tag)

ABIN2181847 产品详细信息, 供应商: Log in to see
蛋白名称
  • OCIF
  • OPG
  • TR1
  • BTF3L1
  • HUMBTFB
  • TNFRSF11B
  • Opg
  • ocif
  • opg
  • tr1
  • tumor necrosis factor receptor superfamily, member 11b
  • basic transcription factor 3 pseudogene 11
  • tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
  • osteoprotegerin
  • TNFRSF11B
  • BTF3P11
  • Tnfrsf11b
  • LOC100037695
  • tnfrsf11b
产品特性
AA 22-401
11
2
2
1
1
1
1
1
宿主

33
4
1
1
1
资源
HEK-293 Cells
9
5
4
3
3
3
2
1
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
标记
His tag
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产品特性 This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 44.4 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/mL (100 μL/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/mL.
背景 Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
分子量 44.4 kDa
NCBI登录号 NP_002537
UniProt O00300
研究领域 Cardiovascular, Atherosclerosis, Extracellular Matrix, Innate Immunity, Cytokines
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 PBS, pH 7.4
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 22-401) (Active) protein (His tag) (ABIN2181847) Human Osteoprotegerin, His Tag on SDS-PAGE under reducing (R) condition. The gel was ...
背景 Luan, Lu, Jiang, Zhang, Wang, Yuan, Zhao, Wang, Wang: "Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 1, pp. 245-52, 2012 (PubMed).

Prichett, Patton, Field, Brun, Emery, Tan, Rieman, McClung, Nadeau, Mooney, Suva, Gowen, Nuttall: "Identification and cloning of a human urea transporter HUT11, which is downregulated during adipogenesis of explant cultures of human bone." in: Journal of cellular biochemistry, Vol. 76, Issue 4, pp. 639-50, 2000 (PubMed).

Emery, McDonnell, Burke, Deen, Lyn, Silverman, Dul, Appelbaum, Eichman, DiPrinzio, Dodds, James, Rosenberg, Lee, Young: "Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14363-7, 1998 (PubMed).

Tsuda, Goto, Mochizuki, Yano, Kobayashi, Morinaga, Higashio: "Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis." in: Biochemical and biophysical research communications, Vol. 234, Issue 1, pp. 137-42, 1997 (PubMed).