ISG15 Protein (AA 2-157) (His tag)
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- 抗原 See all ISG15 蛋白
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-157
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ISG15 protein is labelled with His tag.
- 原理
- Recombinant Human Ubiquitin-Like Protein ISG15/ISG15 (C-6His)
- 序列
- GWDLTVKMLA GNEFQVSLSS SMSVSELKAQ ITQKIGVHAF QQRLAVHPSG VALQDRVPLA SQGLGPGSTV LLVVDKCDEP LNILVRNNKG RSSTYEVRLT QTVAHLKQQV SGLEGVQDDL FWLTFEGKPL EDQLPLGEYG LKPLSTVFMN LRLRGGLEHH HHHH
- 产品特性
- Recombinant Human Ubiquitin-Like Protein ISG15/ISG15 is produced with our E. coli expression system. The target protein is expressed with sequence (Gly2-Gly157) of Human ISG15 fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ISG15 蛋白
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ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-157) protein (CaM)
ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 98 % by SDS - PAGE SDS
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-165) protein (GST tag)ISG15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 1-100) protein (GST tag)ISG15 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-155) protein (His tag)Crystallography grade ISG15 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (GST tag)ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
ISG15 Ubiquitin-Like Modifier (ISG15) (AA 2-157), (full length) protein (His tag)ISG15 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 100 mM NaCl, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))
- 别名
- ISG15 (ISG15 产品)
- 别名
- G1P2 Protein, IFI15 Protein, IP17 Protein, UCRP Protein, hUCRP Protein, ISG15 Protein, g1p2 Protein, si:rp71-1c23.2 Protein, G1p2 Protein, IGI15 Protein, Irfp Protein, ISG17 Protein, ISG17/UCRP Protein, ISG15 ubiquitin-like modifier Protein, ISG15 Protein, isg15 Protein, Isg15 Protein
- Sub Type
- Fusionprotein
- 背景
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Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.
Alternative Names: Ubiquitin-Like Protein ISG15, Interferon-Induced 15 kDa Protein, Interferon-Induced 17 kDa Protein, IP17, Ubiquitin Cross-Reactive Protein, hUCRP, ISG15, G1P2, UCRP - 分子量
- 18.2 kDa
- UniProt
- P05161
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