Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide

ABIN934902 产品详细信息, 供应商: Log in to see
蛋白名称
  • OTTMUSG00000014964
  • Pin1-ps1
  • Pin1l
  • dod
  • ubl5
  • pin1b
  • pin1
  • pin1a
  • DOD
  • UBL5
  • 0610025L01Rik
  • D9Bwg1161e
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, retrogene 1
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • Pin1rt1
  • pin1-b
  • pin1
  • pin1-a
  • PIN1
  • ppiD
  • PITG_11123
  • Pin1
蛋白结构域
AA 22-255, Extracellular Domain
宿主

8
7
7
4
4
4
3
1
1
资源
大肠杆菌(E. Coli)
蛋白类型
Recombinant
生物活性
Active
应用范围
SDS-PAGE (SDS)
选项
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序列 MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
产品特性 Purified recombinant Human Pin 1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 162 nM/min/µg, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.
纯度 > 95 % pure
内毒素水平 < 1.0 EU per µg of protein (determined by LAL method)
背景 Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Alternative Names: Rotamase Pin1 protein, UBL5 protein, PPIase protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein, Pin -1 protein, Pin -1, Pin 1, PPIase Pin1 protein, Pin 1 protein, PPIase Pin1 protein, Rotamase Pin1 protein, DOD protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, PIN1 protein, PIN1 protein, EC 5.2.1.8 protein, Pin 1, EC 5.2.1.8 protein
分子量 18.2 kDa (163 AA)
研究领域 Cell Cycle, Apoptosis/Necrosis
应用备注 Pin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
实验流程
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
    2. Add recombinant Pin 1 protein at various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
    3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
    4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
    5. Record the increase in A410 nm for 20 minutes.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Supplied as a liquid in 20 mM Tris-HCl buffer, pH7.5, containing 0.1 M NaCl, 0 mM DTT, and 20 % glycerol.
储存液 Dithiothreitol (DTT)
注意事项 This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 RT/-20 °C
储存方法 Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
厂商提供的图像
SDS-PAGE (SDS) image for Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) peptide (ABIN934902) Figure annotation denotes ug of protein loaded and % gel used.