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BLUelf Prestained Protein Ladder

ABIN2868250 产品详细信息, 供应商: Log in to see
检测范围
3.5-245 kDa
最低检测浓度
3.5 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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检测方法 Colorimetric
应用备注 Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
说明

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitroce-llulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

试剂准备

Quality Control:
Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

限制 仅限研究用
状态 Liquid
缓冲液 Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM ~3.5 ~5 Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Bis-Tris 10 % Tris-Glycine Urea, and 15 % (v/v) Glycerol). MES buffer 4~20 %
储存条件 RT
储存方法 Store at 25°C for 6 months, Store at -20°C for 24 months
有效期 24 months
厂商提供的图像
 image for BLUelf Prestained Protein Ladder (ABIN2868250) Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis c...