我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PRKAR2B 抗体 (AA 1-418)

Name: PRKAR2B 抗体 (AA 1-418)
SKU: ABIN968073
Availability: OutOfStock

This anti-PRKAR2B antibody is a 小鼠单克隆 antibody detecting PRKAR2B in WB, IHC, IF 和 IP. Suitable for 人, 小鼠, 大鼠, 小鸡和犬. This Primary Antibody has been cited in 5+ publications.

Western Blotting (WB) image for anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibody (ABIN968073) (PRKAR2B 抗体 (AA 1-418))

Western blot analysis of PKA RIIbeta on human endothelial lysate (left). (PRKAR2B 抗体 (AA 1-418))

Immunofluorescent staining of SK-N-SH cells (right). (PRKAR2B 抗体 (AA 1-418))

Image no. 4 for anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibody (ABIN968073) (PRKAR2B 抗体 (AA 1-418))

Immunofluorescence (IF) image for anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibody (ABIN968073) (PRKAR2B 抗体 (AA 1-418))

5 images

(5 references)

产品编号 ABIN968073

发货至: 中国

Datasheet as PDF

Quick Overview for PRKAR2B 抗体 (AA 1-418) (ABIN968073)

抗原

See all PRKAR2B 抗体

PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))

适用

人, 小鼠, 大鼠, 小鸡, 犬

宿主

小鼠

克隆类型

单克隆

标记

This PRKAR2B antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

Discover our top product PRKAR2B Primary Antibody

抗原表位
8

8

8

6

3

3

3

2

2

2

1

1

1

1
AA 1-418

交叉反应

小鸡, 犬, 小鼠, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human PKA RIIbeta, aa 1-418

亚型

IgG1
anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) antibody
PRKAR2B 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibody
PRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Ser113) antibody
PRKAR2B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Internal Region) antibody
PRKAR2B 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 32-62), (N-Term) antibody
PRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB3253 unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibody
PRKAR2B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (pSer113) antibody
PRKAR2B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 304-413) antibody
PRKAR2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (AA 1-418) antibody
PRKAR2B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B) (Center) antibody
PRKAR2B 适用: 人, 小鼠, 大鼠, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Tavalin, Colledge, Hell, Langeberg, Huganir, Scott: "Regulation of GluR1 by the A-kinase anchoring protein 79 (AKAP79) signaling complex shares properties with long-term depression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 8, pp. 3044-51, (2002) (PubMed).

Taskén, Collas, Kemmner, Witczak, Conti, Taskén: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).

Casey, Vaughan, He, Hatcher, Winter, Weremowicz, Montgomery, Kucherlapati, Morton, Basson: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).

Skålhegg, Landmark, Foss, Lohmann, Hansson, Lea, Jahnsen: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).
抗原

PRKAR2B (Protein Kinase, CAMP-Dependent, Regulatory, Type II, beta (PRKAR2B))

别名

PKA RIIbeta

背景

CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIß, RIIalpha, and RIIß.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cß, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rß isoforms are predominant in nervous and adipose tissues. There are indications that the deletion of the gene for PKA RIIß results in lack of long-term potentiation in a select group of hippocampal cells, suggesting an important role for this protein in the neurosciences.

分子量

53 kDa

途径

Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection