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- 抗原 See all Collagen Type V (COL5) 抗体
- Collagen Type V (COL5) (Collagen, Type V (COL5))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen Type V antibody is conjugated to HRP
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Collagen V has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Collagen Type V from human and bovine placenta
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL5 Primary Antibody
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anti-Collagen, Type V (COL5) antibody
COL5 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 1450-1500) antibodyCOL5 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type V (COL5) (AA 61-90), (N-Term) antibodyCOL5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (FITC)COL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal FITC
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人, Cow, 哺乳动物 ELISA, WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:50 to 1:200
Application Note: Anti-Collagen V Peroxidase Conjugated Antibody was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Collagen V in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200.
Western Blot Dilution: 1:5,000 to 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:4,000 to 1:8,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL to 20 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- 有效期
- 12 months
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- 抗原
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- 别名
- Collagen Type V (COL5 产品)
- 背景
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Synonyms: COL5A2 protein antibody, Col5A3 antibody, Collagen fetal membrane A polypeptide antibody
Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
Gene Name: COL5A1
- 基因ID
- 50509
- UniProt
- P25940
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