Antibody AP09481PU detects endogenous levels of TrkB only when phosphorylated at Tyrosine 515.
交叉反应 (详细)
Species reactivity (tested):Human, Mouse and Rat
纯化方法
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from Human TrkB around the phosphorylation site of Tyrosine 515 (P-Q-Y-pF-G).
Immunofluorescence: 1/100approx. 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody (in aliquots) at-20 °C.
抗原
TRKB (NTRK2)
(Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
Neurotrophins activate tyrosine kinase receptors of the Trk family (TrkA, TrkB and TrkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.Synonyms: BDNF/NT-3 growth factors receptor, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKB, TrkB tyrosine kinase