GLI2 抗体 (Isoform alpha)
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- 抗原 See all GLI2 抗体
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
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抗原表位
- AA 40-60, AA 46-60, Isoform alpha
- 适用
- 人, 小鼠, 大鼠, Chimpanzee, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLI2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against Gli-2 protein. Expect reactivity with all isoforms of this protein.
- 交叉反应 （详细）
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Species reactivity (expected):Chimpanzee (100 %), Mouse and Rat (93 %), Bovine (86 %).
Species reactivity (tested):Human. - 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).
- 亚型
- IgG
- Top Product
- Discover our top product GLI2 Primary Antibody
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anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody
GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1287-1321), (C-Term) antibodyGLI2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB49147 unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibodyGLI2 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibodyGLI2 适用: 人, Chimpanzee WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (C-Term) antibodyGLI2 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 600-649) antibodyGLI2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 2-20) antibodyGLI2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (N-Term) antibodyGLI2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1444-1463), (C-Term) antibodyGLI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/2,000-1/12,000. Western blot: 1/500-1/2,000. Detection of Gli-2 may be enhanced if nuclear extracts areused instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced,making it difficult to detect in whole tissue homogenates. Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.1 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
- 别名
- GLI2 (GLI2 产品)
- 别名
- HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody
- 背景
- Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is a nuclear protein thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes, Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2
- 基因ID
- 2736
- NCBI登录号
- NP_005261
- UniProt
- P10070
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis
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