HA-标记 抗体

ABIN387848 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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宿主

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克隆类型 (克隆位点)
多克隆 ()
标记
非结合性
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应用范围
Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Western Blotting (WB)
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选项
Supplier
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免疫原 KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
序列 YPYDVPDYA
克隆位点 RB0411
亚型 Ig
特异性 KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
物质类 Tag
背景 Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Synonyms: Tag from influenza hemagglutinin protein
分子量 DA
研究领域 Tags/Labels
应用备注 WB = 1:1000, IF = 1:10-50, CHIP = 1:100
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for anti-HA-Tag antibody (ABIN387848) Western blot analysis of anti-HA tag Pab (ABIN387848)in HA-tagged recombinant protein...
Immunohistochemistry (IHC) image for anti-HA-Tag antibody (ABIN387848) GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 f...
Immunohistochemistry (IHC) image for anti-HA-Tag antibody (ABIN387848) ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune ...
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Han, Guan, Wang, Hatzoglou, Mu: "L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated ?1(D219N) Subunits of GABAA Receptors." in: ACS chemical biology, 2015 (PubMed).

Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, 2015 (PubMed).

Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." in: Journal of biochemistry, 2015 (PubMed).

Oh, Yue, Wang, Tong, Chen, Khuri, Sun: "Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling." in: Oncotarget, 2015 (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." in: Journal of integrative plant biology, 2015 (PubMed).

Zhao, Yue, Khuri, Sun: "mTOR complex 2 is involved in regulation of Cbl-dependent c-FLIP degradation and sensitivity of TRAIL-induced apoptosis." in: Cancer research, Vol. 73, Issue 6, pp. 1946-57, 2013 (PubMed).

Oh, Yue, Zhou, Balko, Black, Owonikoko, Khuri, Sun: "Oncogenic Ras and B-Raf proteins positively regulate death receptor 5 expression through co-activation of ERK and JNK signaling." in: The Journal of biological chemistry, Vol. 287, Issue 1, pp. 257-67, 2012 (PubMed).

Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, 2012 (PubMed).

Zhao, Yue, Lonial, Khuri, Sun: "The NEDD8-activating enzyme inhibitor, MLN4924, cooperates with TRAIL to augment apoptosis through facilitating c-FLIP degradation in head and neck cancer cells." in: Molecular cancer therapeutics, Vol. 10, Issue 12, pp. 2415-25, 2011 (PubMed).

Fan, Li, Yue, Khuri, Sun: "The eIF4E/eIF4G interaction inhibitor 4EGI-1 augments TRAIL-mediated apoptosis through c-FLIP Down-regulation and DR5 induction independent of inhibition of cap-dependent protein translation." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 4, pp. 346-56, 2010 (PubMed).

Guo, Wang, Wang, Huang, Liang, Chen: "Dissection of the relationship between RACK1 and heterotrimeric G-proteins in Arabidopsis." in: Plant & cell physiology, Vol. 50, Issue 9, pp. 1681-94, 2009 (PubMed).

Scarselli, Donaldson: "Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3577-85, 2009 (PubMed).

Lin, Liu, Yue, Benbrook, Berlin, Khuri, Sun: "Involvement of c-FLIP and survivin down-regulation in flexible heteroarotinoid-induced apoptosis and enhancement of TRAIL-initiated apoptosis in lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 11, pp. 3556-65, 2008 (PubMed).

Wang, Kwak, Zeng, Ellis, Chen, Schiefelbein, Chen: "TRICHOMELESS1 regulates trichome patterning by suppressing GLABRA1 in Arabidopsis." in: Development (Cambridge, England), Vol. 134, Issue 21, pp. 3873-82, 2007 (PubMed).

Zemskov, Mikhailenko, Strickland, Belkin: "Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1." in: Journal of cell science, Vol. 120, Issue Pt 18, pp. 3188-99, 2007 (PubMed).

Liu, Yue, Schönthal, Khuri, Sun: "Cellular FLICE-inhibitory protein down-regulation contributes to celecoxib-induced apoptosis in human lung cancer cells." in: Cancer research, Vol. 66, Issue 23, pp. 11115-9, 2006 (PubMed).

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