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HA-Tag 抗体

WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN100176
发货至: 中国
  • 抗原 See all HA-Tag products
    HA-Tag
    适用
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    宿主
    • 74
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    克隆类型
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    多克隆
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    This HA-Tag antibody is un-conjugated
    应用范围
    • 133
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    序列
    YPYDVPDYA
    特异性
    This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
    产品特性
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
    过滤
    Sterile filtered
    免疫原
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
    Immunogen Type: Peptide
    亚型
    IgG
  • 应用备注
    Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.95 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    有效期
    12 months
  • Wallis, Ventimiglia, Otigbah, Infante, Cuesta-Geijo, Kidiyoor, Carbajal, Fleck, Foiani, Garcia-Manyes, Martin-Serrano, Agromayor: "The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair." in: Developmental cell, Vol. 56, Issue 23, pp. 3192-3202.e8, (2021) (PubMed).

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    Pati, Salvi, Kallianpur, Vaidya, Banerjee, Maiti, Clement, Vaidya: "Chemogenetic Activation of Excitatory Neurons Alters Hippocampal Neurotransmission in a Dose-Dependent Manner." in: eNeuro, Vol. 6, Issue 6, (2020) (PubMed).

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    Ventimiglia, Cuesta-Geijo, Martinelli, Caballe, Macheboeuf, Miguet, Parnham, Olmos, Carlton, Weissenhorn, Martin-Serrano: "CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope." in: Developmental cell, Vol. 47, Issue 5, pp. 547-563.e6, (2019) (PubMed).

    Yamada, Murata, Adachi, Itoh, Kameoka, Igarashi, Kato, Araki, Huganir, Dawson, Yanagawa, Okamoto, Iijima, Sesaki: "Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease." in: Cell metabolism, Vol. 28, Issue 4, pp. 588-604.e5, (2019) (PubMed).

    Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

    Kijima, Prince, Tigue, Yim, Schwartz, Beebe, Lee, Budzynski, Williams, Trepel, Sistonen, Calderwood, Neckers: "HSP90 inhibitors disrupt a transient HSP90-HSF1 interaction and identify a noncanonical model of HSP90-mediated HSF1 regulation." in: Scientific reports, Vol. 8, Issue 1, pp. 6976, (2018) (PubMed).

    Ulu, Oh, Zuo, Frost: "Stress-activated MAPKs and CRM1 regulate the subcellular localization of Net1A to control cell motility and invasion." in: Journal of cell science, Vol. 131, Issue 3, (2018) (PubMed).

    Bohn, Thummar, York, Raymond, Brown, Bieniasz, Hatziioannou, Smith: "APOBEC3H structure reveals an unusual mechanism of interaction with duplex RNA." in: Nature communications, Vol. 8, Issue 1, pp. 1021, (2018) (PubMed).

    Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

    Kim, Wang, Sanchez, Coukos, von Zastrow, Ting: "Time-gated detection of protein-protein interactions with transcriptional readout." in: eLife, Vol. 6, (2018) (PubMed).

    Kanter, Lykken, Avesar, Weible, Dickinson, Dunn, Borgesius, Roudi, Kentros: "A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II." in: Neuron, Vol. 93, Issue 6, pp. 1480-1492.e6, (2017) (PubMed).

    York, Kutluay, Errando, Bieniasz: "The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid." in: PLoS pathogens, Vol. 12, Issue 8, pp. e1005833, (2017) (PubMed).

    Blanco-Melo, Gifford, Bieniasz: "Co-option of an endogenous retrovirus envelope for host defense in hominid ancestors." in: eLife, Vol. 6, (2017) (PubMed).

    Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

  • 抗原
    HA-Tag
    别名
    HA tag (HA-Tag 产品)
    物质类
    Tag
    背景
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
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