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HA-标记 抗体 (HRP)

ABIN387712 产品详细信息, 供应商: Log in to see
抗原
适用
标记
450
2
1
1
1
1
1
宿主
小鼠
180
177
40
34
20
3
克隆类型 (克隆位点)
单克隆 ()
标记
HRP
42
29
23
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
360
200
115
109
81
70
53
40
15
13
11
7
7
5
4
4
4
4
3
2
2
1
1
1
1
1
1
Supplier
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品牌 THE™
免疫原 A synthetic peptide (YPYDVPDYA) coupled - KLH
序列 YPYDVPDYA
克隆位点 5E11D8
亚型 IgG
特异性 THETM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.
产品特性 The antibody is suitable for detecting the expression level of HA in fusion proteins. Do not add azide as it inhibits HRP activity.
纯化方法 Protein A affinity column
别名 HA Tag
物质类 Tag
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-0.5 µg/mL
Western blot: 0.1-0.5 µg/mL
Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
浓度 0.5 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
 image for anti-HA-标记 抗体 (HRP) (ABIN387712) Lane 1-3: 2 µL, 0.4 µL, 0.08 µL N-terminal HA-GST fusion protein in E. coli cell lysa...
 image for anti-HA-标记 抗体 (HRP) (ABIN387712) 293 cells transfected with N-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
 image for anti-HA-标记 抗体 (HRP) (ABIN387712) 293 cells transfected with C-terminal HA tag protein. Primary antibody: 1 µg/mL Anti-...
有引用在: Haac, Anderson, Eggleston, Myles, Adelman: "The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3688-700, 2015 (PubMed).