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His 标记 抗体 (HRP)

ABIN387701 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
18
3
1
宿主
小鼠
397
178
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
HRP
57
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Western Blotting (WB)
528
371
160
106
77
75
66
26
12
11
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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品牌 THE™
免疫原 A synthetic peptide HHHHHH coupled - KLH
序列 HHHHHH
克隆位点 6G2A9
亚型 IgG
特异性 THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
产品特性 This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
纯化方法 Protein A affinity column
物质类 Tag
研究领域 Tags/Labels
应用备注 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
浓度 0.5 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing cycles.
储存条件 4 °C/-20 °C
储存方法 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
厂商提供的图像
 image for anti-His 标记 抗体 (HRP) (ABIN387701) Lane 1: MarkerLane 2: 10 ng Multiple Tag Cell Lysate fusion protein (ABIN1536505) Lan...
有引用在: Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." in: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, 2015 (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." in: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, 2015 (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." in: PLoS ONE, Vol. 9, Issue 9, pp. e107683, 2014 (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." in: Cancer research, Vol. 74, Issue 24, pp. 7510-20, 2014 (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." in: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, 2013 (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." in: Microbial cell factories, Vol. 11, pp. 118, 2012 (PubMed).