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小鼠 anti-人 IgE (Fc Region) Antibody

ABIN363703 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Fc Region
135
60
53
7
1
1
1
1
1
适用

484
141
46
44
6
6
3
2
2
2
1
1
1
宿主
小鼠
291
286
67
52
10
6
3
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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免疫原 purified human myeloma IgE
克隆位点 BSW17-NBS01
亚型 IgG1 kappa
特异性 Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
产品特性 IGHE, chromosome 14q32.33
纯化方法 Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
纯度 > 95 %, tested by analytical Gel Permeation Chromatography
背景 The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
研究领域 Immunology, Secondary Antibodies
应用备注 When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
限制 仅限研究用
浓度 1 mg/mL
缓冲液 PBS, pH=7.2, 0.1 % NaN3
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
储存条件 4 °C
厂商提供的图像
 image for 小鼠 anti-人 IgE (Fc Region) Antibody (ABIN363703) product purity: gel permeation chromatography (Superose 12/HR)
 image for 小鼠 anti-人 IgE (Fc Region) Antibody (ABIN363703) Target binding kinetics (Biacore
有引用在: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

背景 Rudolf, Furukawa, Miescher, Vogel, Kricek, Stadler: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Czech, Stadler, Schuopf, Kapp: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).