小鼠 anti-人 IgE (Fc Region) Antibody

ABIN363692 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Fc Region
135
60
53
7
1
1
1
1
1
适用

489
141
46
44
6
6
3
2
2
2
1
1
1
宿主
小鼠
296
286
67
52
10
6
3
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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免疫原 purified human myeloma IgE
克隆位点 Le27-NBS01
亚型 IgG1 kappa
特异性 Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
纯化方法 Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
纯度 > 95 %, tested by analytical Gel Permeation Chromatography
背景 The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.

Gene: IGHE, chromosome 14q32.33
研究领域 Immunology, Secondary Antibodies
应用备注 For functional cellular assays (mast cell / basophil activation), when used at dilutions 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
限制 仅限研究用
浓度 1 mg/mL
缓冲液 PBS, pH = 7.2, 0.1 % NaN3
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
储存条件 4 °C
厂商提供的图像
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363692) product purity: gel permeation chromatography (Superose 12/HR)
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363692) Target binding kinetics (Biacore
有引用在: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

背景 Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).